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Article

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies.

Biotechnology journal - 1 Mar 2016

Chan Kah Fai, Shahreel Wahyu, Wan Corrine, Teo Gavin, Hayati Noor, Tay Shi Jie, Tong Wen Han, Yang Yuansheng, Rudd Pauline M, Zhang Peiqing, Song Zhiwei

Abstract excerpt

Removal of core fucose from N-glycans attached to human IgG1 significantly enhances its affinity for the receptor FcγRIII and thereby dramatically improves its antibody-dependent cellular cytotoxicity activity. While previous works have shown that inactivation of fucosyltransferase 8 results in mutants capable of producing fucose-free antibodies, we report here the use of genome editing techniques, namely ZFNs,...

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PMID: 26471004
DOI: 10.1002/biot.201500331

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EPO

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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