Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Highly efficient deletion of <i>FUT8</i> in CHO cell lines using zinc‐finger nucleases yields cells that produce completely nonfucosylated antibodies

8 Apr 2010

Abstract excerpt

IgG1 antibodies produced in Chinese hamster ovary (CHO) cells are heavily alpha1,6-fucosylated, a modification that reduces antibody-dependent cellular cytotoxicity (ADCC) and can inhibit therapeutic antibody function in vivo. Addition of fucose is catalyzed by Fut8, a alpha1,6-fucosyltransferase. FUT8(-/-) CHO cell lines produce completely nonfucosylated antibodies, but the difficulty of recapitulating the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Monoclonal and Polyclonal Antibodies Research
Share to Transgenic Plants and Applications
Share to Viral Infectious Diseases and Gene Expression in Insects

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 20564614
DOI: 10.1002/bit.22751

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Highly efficient deletion of <i>FUT8</i> in CHO cell lines using zinc‐finger nucleases yields cells that produce completely nonfucosylated antibodiesPMID 20564614Read ↗

Producing defucosylated antibodies with enhanced in vitro antibody‐dependent cellular cytotoxicity via FUT8 knockout CHO‐S cellsSemantic proximity Read ↗

Establishment of FUT8 gene knockout CHO cell line with stable expression of monoclonal antibodySemantic proximity Read ↗

Functional knockout of FUT8 in Chinese hamster ovary cells using CRISPR/Cas9 to produce a defucosylated antibodySemantic proximity Read ↗

Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity.Semantic proximity Read ↗

FX knockout CHO hosts can express desired ratios of fucosylated or afucosylated antibodies with high titers and comparable product qualitySemantic proximity Read ↗

Inactivation of GDP-fucose transporter gene (Slc35c1) in CHO cells by ZFNs, TALENs and CRISPR-Cas9 for production of fucose-free antibodies.Semantic proximity Read ↗

Accelerated generation of gene‐engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9Semantic proximity Read ↗

Enhancing recombinant antibody yield in Chinese hamster ovary cellsSemantic proximity Read ↗

Biallelic gene knockouts in Chinese hamster ovary cells.Semantic proximity Read ↗

Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cellsSemantic proximity Read ↗

Functional diversification of hybridoma produced antibodies by CRISPR/HDR genomic engineeringSemantic proximity Read ↗

Glycoengineering design options for IgG1 in CHO cells using precise gene editingSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.