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The interplay of protein engineering and glycoengineering to fine-tune antibody glycosylation and its impact on effector functions.

Biotechnology and bioengineering - 1 Jan 2022

Wang Qiong, Wang Tiexin, Zhang Roushu, Yang Shuang, McFarland Kevin S, Chung Cheng-Yu, Jia Hongpeng, Wang Lai-Xi, Cipollo John F, Betenbaugh Michael J

Abstract excerpt

The N-glycan pattern of an IgG antibody, attached at a conserved site within the fragment crystallizable (Fc) region, is a critical antibody quality attribute whose structural variability can also impact antibody function. For tailoring the Fc glycoprofile, glycoengineering in cell lines as well as Fc amino acid mutations have been applied. Multiple glycoengineered Chinese hamster ovary cell lines were generated,...

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PMID: 34647616
DOI: 10.1002/bit.27953

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