Article
Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity.
Biotechnology and bioengineering - 5 Sept 2004
Yamane-Ohnuki Naoko, Kinoshita Satoko, Inoue-Urakubo Miho, Kusunoki Machi, Iida Shigeru, Nakano Ryosuke, Wakitani Masako, Niwa Rinpei, Sakurada Mikiko, Uchida Kazuhisa, Shitara Kenya, Satoh Mitsuo
Abstract excerpt
To generate industrially applicable new host cell lines for antibody production with optimizing antibody-dependent cellular cytotoxicity (ADCC) we disrupted both FUT8 alleles in a Chinese hamster ovary (CHO)/DG44 cell line by sequential homologous recombination. FUT8 encodes an alpha-1,6-fucosyltransferase that catalyzes the transfer of fucose from GDP-fucose to N-acetylglucosamine (GlcNAc) in an alpha-1,6...
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