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Accelerated generation of gene‐engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9

1 Apr 2024

Abstract excerpt

Chinese hamster ovary (CHO) cells are the commonly used mammalian host system to manufacture recombinant proteins including monoclonal antibodies. However unfavorable non-human glycoprofile displayed on CHO-produced monoclonal antibodies have negative impacts on product quality, pharmacokinetics, and therapeutic efficiency. Glycoengineering such as genetic elimination of genes involved in glycosylation pathway in...

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PMID: 38651269
DOI: 10.1002/biot.202300505

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