Article

Rapid selection of transgenic mammalian cells via diphtheria toxin resistance

2024-08-26

Abstract excerpt

The ability to generate stable transgenic mammalian cell lines is crucial to the investigation of gene functions and the production of recombinant proteins. Currently, mammalian cells can be readily transfected in cell culture settings via both viral and nonviral vectors to induce transgene expression. However, there is an unmet need for efficient selection of transfected cells, since current methods involve rathe...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 9e510019-cec0-51ea-a79b-aab3e666c5a6
DOI: 10.1101/2024.08.26.609662

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Rapid selection of transgenic mammalian cells via diphtheria toxin resistanceDOI 10.1101/2024.08.26.609662Read

Universal toxin-based selection for precise genome engineering in human cells.Semantic proximity Read

Transgenic mice expressing the diphtheria toxin receptor are sensitive to the toxinSemantic proximity Read

Mutations in diphtheria toxin to improve immunotoxin selectivity and understand toxin entry into cells.Semantic proximity Read

The Terminator mouse is a diphtheria toxin-receptor knock-in mouse strain for rapid and efficient enrichment of desired cell lineages.Semantic proximity Read

Suppression of single and double nonsense mutations introduced into the diphtheria toxin A-chain gene: a potential binary system for toxin gene therapy.Semantic proximity Read

Targeted introduction of a diphtheria toxin-resistant point mutation into the chromosomal EF-2 locus by in vivo homologous recombination.Semantic proximity Read

Transgenic mice expressing a fully nontoxic diphtheria toxin mutant, not CRM197 mutant, acquire immune tolerance against diphtheria toxin.Semantic proximity Read

Engineering immunotoxin-equipped effector cells and evaluation in primary human immune cellsSemantic proximity Read

In vivo selection using a cell-growth switch.Semantic proximity Read

Detection of mutation in transgenic CHO cells using green fluorescent protein as a reporter.Semantic proximity Read

Retroviral insertional mutagenesis identifies a small protein required for synthesis of diphthamide, the target of bacterial ADP-ribosylating toxins.Semantic proximity Read

Enhanced ouabain resistance gene as a eukaryotic selection marker.Semantic proximity Read