Article

RheoScale 2.0: Revealing the Hidden Roles of Protein Positions via Substitution Patterns

2026-08-11

Abstract excerpt

A central challenge in molecular biology is understanding how amino acid substitutions modulate various features of protein function and stability. To illuminate the complexities of this relationship, high-throughput (HTP) assays are increasingly used to assess site-saturating mutagenesis libraries. A common downstream analysis is to average the set of twenty outcomes at each amino acid position for comparison wit...

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Literature Corpus work: 995b16db-028d-5af6-bae9-e3976ca431c4
DOI: 10.64898/2026.08.10.743964

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