Article

Accurate Determination of Human CPR Conformational Equilibrium by smFRET using Dual Orthogonal Non-Canonical Amino Acid Labeling

2018-09-04

Abstract excerpt

<h4>ABSTRACT</h4> Conjugation of fluorescent dyes to proteins - a prerequisite for the study of conformational dynamics by single molecule Förster resonance energy transfer (smFRET) - can lead to substantial changes of the dye’s photophysical properties, ultimately biasing the quantitative determination of inter-dye distances. In particular the popular cyanine dyes and their derivatives, which are by far the most...

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Literature Corpus work: 987d6449-b112-520a-86e5-267e1ddb90f7
DOI: 10.1101/407684

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