Article
Selective Double-Labeling of Cell-Free Synthesized Proteins for More Accurate smFRET Studies.
Analytical chemistry - 7 Nov 2017
Sadoine Mayuri, Cerminara Michele, Kempf Noémie, Gerrits Michael, Fitter Jörg, Katranidis Alexandros
Abstract excerpt
Förster resonance energy transfer (FRET) studies performed at the single molecule level have unique abilities to probe molecular structure, dynamics, and function of biological molecules. This technique requires specimens, like proteins, equipped with two different fluorescent probes attached at specific positions within the molecule of interest. Here, we present an approach of cell-free protein synthesis (CFPS)...
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