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PHYCUT: Scalable multiplex CRISPR/Cas9 editing for genome engineering in the diatom <i>Phaeodactylum tricornutum</i>

2025-10-31

Abstract excerpt

<h4>ABSTRACT</h4> Diatoms are globally significant microalgae that contribute ∼ 20% of oxygen production and exhibit remarkable metabolic diversity. The marine diatom Phaeodactylum tricornutum has emerged as a promising synthetic biology platform for bioproduction of recombinant proteins, supported by a human-like N -linked glycosylation pathway. However, its α (1,3)-linked core fucose is immunogenic in humans...

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Literature Corpus work: 979cec62-65b1-5d47-84cf-9af8f33f69b8
DOI: 10.1101/2025.10.31.685893

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