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A Streamlined Protocol for Small-scale Protoplast Generation and CRISPR/Cpf1-mediated Genome Editing in <i>Fusarium oxysporum</i>

2026-02-02

Abstract excerpt

<h4>SUMMARY</h4> Fusarium oxysporum is a significant threat to agriculture and One Health, requiring advanced molecular tools for functional genomics analyses and biological control agent development. Existing gene-editing methods are hampered by costly protoplast preparation protocols and by CRISPR/Cas9 limitations, such as restricted PAM sequences and complex guide RNA requirements. We engineered an efficient C...

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Literature Corpus work
06906514-6a23-5969-bf75-e8c1f40744b3
DOI
10.64898/2026.01.28.702186
Open publication

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A Streamlined Protocol for Small-scale Protoplast Generation and CRISPR/Cpf1-mediated Genome Editing in <i>Fusarium oxysporum</i>DOI 10.64898/2026.01.28.702186
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