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Inactivation of the β (1, 2)-xylosyltransferase and the α (1, 3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy.

Plant cell reports - 1 Jun 2021

Jung Jae-Wan, Shin Jun-Hye, Lee Won-Kyung, Begum Hilal, Min Chan-Hong, Jang Mi-Hwa, Oh Han-Bin, Yang Moon-Sik, Kim Seong-Ryong

Abstract excerpt

KEY MESSAGE: CRISPR/Cas9-mediated OsXylT and OsFucT mutation caused the elimination of plant-specific β1,2-xylose and α1,3-fucose residues on glycoproteins in rice, which is the first report of OsXylT/OsFucT double KO mutation in rice. N-glycosylation pathway is the one of post-translational mechanism and is known as highly conserved in eukaryotes. However, the process for complex-N-glycan modification is...

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PMID: 33547931
DOI: 10.1007/s00299-021-02667-8

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