Article

Chemical probe-based Nanopore Sequencing to Selectively Assess the RNA modifications

2020-05-20

Abstract excerpt

Current methods to identify RNA modifications with short-read sequencing are laborious and direct RNA sequencing gets proclaimed as the viable alternative. Herein, we harness the selective reactivity of the acrylonitrile towards the Inosine ( I) and pseudouridine ( Ψ ) modifications and developed a chemical probe-based direct RNA sequencing method. We first demonstrated that the chemical probe-induced differenti...

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Literature Corpus work: 8fadedab-4de3-5385-8e55-d00ba8f6db3d
DOI: 10.1101/2020.05.19.105338

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