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Article

Thermodynamically programmed one-pot CRISPR platform for point-of-care SNP genotyping.

Nature communications - 7 Jul 2026

Wu Xiaolong, Li Yanan, Cao Yumeng, Zhao Zibin, Lu Hongyu, Liang Shaochong, Lui Grace C Y, Chan Denise P C, Hsing I-Ming

Abstract excerpt

One-pot CRISPR diagnostics face a fundamental incompatibility: isothermal nucleic acid amplification enables rapid target accumulation, whereas CRISPR activation irreversibly consumes those substrates, destabilizing reaction kinetics. Here we show that reaction order can be programmed into DNA primers through thermodynamic design. Differences in primer-binding strength create two sequential amplification stages,...

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Share to Clustered Regularly Interspaced Short Palindromic Repeats
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 42414323
DOI: 10.1038/s41467-026-75358-1
PMCID: PMC13473493

Genes

PAM

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

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