Article

The effect of variant interference on <i>de novo</i> assembly for viral deep sequencing

2019-10-22

Abstract excerpt

Viruses have high mutation rates and generally exist as a mixture of variants in biological samples. Next-generation sequencing (NGS) approach has surpassed Sanger for generating long viral sequences, yet how variants affect NGS de novo assembly remains largely unexplored. Our results from >15,000 simulated experiments showed that presence of variants can turn an assembly of one genome into tens to thousands of c...

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Literature Corpus work: 8bd29a0e-f336-50b7-9bd6-eda8017c47c4
DOI: 10.1101/815480

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