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Article

The effect of variant interference on de novo assembly for viral deep sequencing.

BMC genomics - 22 Jun 2020

Castro Christina J, Marine Rachel L, Ramos Edward, Ng Terry Fei Fan

Abstract excerpt

BACKGROUND: Viruses have high mutation rates and generally exist as a mixture of variants in biological samples. Next-generation sequencing (NGS) approaches have surpassed Sanger for generating long viral sequences, yet how variants affect NGS de novo assembly remains largely unexplored. RESULTS: Our results from > 15,000 simulated experiments showed that presence of variants can turn an assembly of one genome...

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Identifiers

PMID: 32571214
DOI: 10.1186/s12864-020-06801-w
PMCID: PMC7306937

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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