A universal, high-quality, and high-yield DNA purification method for mycobacteria, including Mycobacterium tuberculosis : large-scale assessment of the chloroform-bead method

Abstract excerpt

ABSTRACT Genomic analysis of mycobacteria has become increasingly crucial for understanding drug-resistance mechanisms, molecular epidemiology, and pathogenesis. However, efficient extraction of high-molecular-weight genomic DNA from these organisms remains challenging because of their thick mycolic acid-rich cell walls. In this study, we report the chloroform-bead method, a universal DNA extraction protocol that...

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