Article

Targeted protein degradation using deGradFP in Trypanosoma brucei

2022-10-19

Abstract excerpt

Targeted protein degradation is an invaluable tool in studying the function of proteins. Such a tool was not available in Trypanosoma brucei , an evolutionarily divergent eukaryote that causes human African trypanosomiasis. Here, we have adapted deGradFP (degrade green fluorescent protein [GFP]), a protein degradation system based on the SCF E3 ubiquitin ligase complex and anti-GFP nanobody, in T. brucei . As a...

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Literature Corpus work: 896d5b89-7645-51b6-9dde-8ef9283cac2f
DOI: 10.12688/wellcomeopenres.17964.2

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