Article
iCODA: RNAi-based inducible knock-in system in Trypanosoma brucei.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2011
Ringpis Gene-Errol, Lathrop Richard H, Aphasizhev Ruslan
Abstract excerpt
In vivo mutational analysis is often required to characterize enzymes that function as subunits of the U-insertion/deletion RNA editing core complex (RECC) in mitochondria of Trypanosoma brucei. The mutations may skew phenotypic manifestation of a dominant negative overexpression if complex association is disrupted. Conditional knockouts and knock-ins of essential mitochondrial genes are time consuming and...
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