Article

Cell-Type-Resolved Isoform Atlas of Human Tissues Reveals Age and Alzheimer’s Disease-Associated Splicing Changes

2025-11-16

Abstract excerpt

Alternative splicing is a key mechanism for transcriptomic diversity, but how isoforms map to specific cell types in bulk tissues remains unclear. We present Sciege, a multimodal method that integrates bulk short-read RNA-seq with single-cell and long-read data to estimate cell type-specific isoform distributions. Through simulations, we demonstrate that Sciege accurately estimates isoform abundances and identifie...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 2a7d14bc-b928-551b-8c91-e0bd3aae76eb
DOI: 10.1101/2025.11.14.688520

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Cell-Type-Resolved Isoform Atlas of Human Tissues Reveals Age and Alzheimer’s Disease-Associated Splicing ChangesDOI 10.1101/2025.11.14.688520Read

SCOTCH: isoform-level characterization of gene expression through long-read single-cell RNA sequencingSemantic proximity Read

Single-cell splicing analysis with <i>ISSAC</i> links cell type-specific and cell state-dependent sQTLs to neurological disordersSemantic proximity Read

Using deep long-read RNAseq in Alzheimer’s disease brain to assess medical relevance of RNA isoform diversitySemantic proximity Read

Accurate strand-specific long-read transcript isoform discovery and quantification at bulk, single-cell, and single-nucleus resolutionSemantic proximity Read

Surveying the landscape of RNA isoform diversity and expression across 9 GTEx tissues using long-read sequencing dataSemantic proximity Read

Analyzing cell-type-specific isoform expression using IsoDiffR and long-read single-cell RNA sequencingSemantic proximity Read

Single-cell atlas of transcriptomic vulnerability across multiple neurodegenerative and neuropsychiatric diseasesSemantic proximity Read

RNA isoform diversity, splicing variants, and switching in single cells of the Alzheimer’s disease brainSemantic proximity Read

A Single-cell and Spatial RNA-seq Database for Alzheimer’s Disease (ssREAD)Semantic proximity Read

Cell-type specific inference from bulk RNA-sequencing data by integrating single cell reference profiles via EPIC-unmixSemantic proximity Read

BrainBridge Characterizes Key Factors affecting Alzheimer’s Disease and Associated PhenotypesSemantic proximity Read

Hypatia: Comparative Isoform Profiling Across Cell Populations from Long-Read Single-Cell TranscriptomesSemantic proximity Read