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Agarose Gel Microcapsules Enable Easy-to-prepare, Picolitre-scale Single-cell Genomics, Yielding Near-complete Genome Sequences

2022-02-03

Abstract excerpt

A novel type of agarose gel microcapsule (AGM), consisting of an alginate picolitre sol core and an agarose gel shell, was developed to obtain high-quality single-cell amplified genomic DNA of bacteria. The AGM is easy to prepare in a stable emulsion with oil of water-equivalent density which prevents AGM aggregation, with only standard laboratory equipment. Single cells from a pure culture of Escherichia coli , a...

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Literature Corpus work: 8646a050-8ba7-539e-a628-64d9fa219f48
DOI: 10.21203/rs.3.rs-147972/v2

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