Nearly finished genomes produced using gel microdroplet culturing reveal substantial intraspecies genomic diversity within the human microbiome.

doi:10.1101/gr.142208.112

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Article

Nearly finished genomes produced using gel microdroplet culturing reveal substantial intraspecies genomic diversity within the human microbiome.

Genome research - 1 May 2013

Fitzsimons Michael S, Novotny Mark, Lo Chien-Chi, Dichosa Armand E K, Yee-Greenbaum Joyclyn L, Snook Jeremy P, Gu Wei, Chertkov Olga, Davenport Karen W, McMurry Kim, Reitenga Krista G, Daughton Ashlynn R, He Jian, Johnson Shannon L, Gleasner Cheryl D, Wills Patti L, Parson-Quintana Beverly, Chain Patrick S, Detter John C, Lasken Roger S, Han Cliff S

Abstract excerpt

The majority of microbial genomic diversity remains unexplored. This is largely due to our inability to culture most microorganisms in isolation, which is a prerequisite for traditional genome sequencing. Single-cell sequencing has allowed researchers to circumvent this limitation. DNA is amplified directly from a single cell using the whole-genome amplification technique of multiple displacement amplification...

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PMID: 23493677
DOI: 10.1101/gr.142208.112
PMCID: PMC3638143

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