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Article

Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes.

Scientific reports - 1 Feb 2018

Kogawa Masato, Hosokawa Masahito, Nishikawa Yohei, Mori Kazuki, Takeyama Haruko

Abstract excerpt

Single-cell genomics is a straightforward approach to obtain genomes from uncultured microbes. However, sequence reads from a single-cell amplified genome (SAG) contain significant bias and chimeric sequences. Here, we describe Cleaning and Co-assembly of a Single-Cell Amplified Genome (ccSAG), a novel analytical workflow to obtain composite single-cell genomes with elimination of sequence errors. By the...

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Identifiers

PMID: 29391438
DOI: 10.1038/s41598-018-20384-3
PMCID: PMC5794965

Genes

SAG

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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