Article
Direct visualization of living bacterial genotypes using CRISPR/Cas12a-circular reporter nanoprobes.
Biosensors & bioelectronics - 15 Nov 2022
Li Yanan, Pei Yiran, Huang Di, Xu Ru, Wu Yonghua, Li Yaqiong, Zhou Ruixue, Wei Yongbin, Tan Xiao, Gao Hua, Shi Jinjin, Zhang Zhenzhong, Zhang Kaixiang, Li Jinghong
Abstract excerpt
Bacterial genotyping is important for understanding the complex microbiota. Although fluorescence in situ hybridization (FISH) has enabled bacterial community identification with high spatial resolution, its unavoidable cell fixation steps and signal generation by multi-probe stacking greatly limit its application in living bacterial genotyping. Here, we designed polyethyleneimine-encapsulated...
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