Article

Homogeneous label-free fluorescent assay of small molecule-protein interactions using protein binding-inhibited transcription nanomachine.

2011-08-01

Abstract excerpt

Quantitative analysis of interactions between small molecules and proteins is a central challenge in chemical genetics, molecular diagnostics and drug developments. Here, we developed a RNA transcription nanomachine by assembling T7 RNA polymerase on a small molecule-labeled DNA heteroduplex. The nanomachine, of which the RNA transcription activity can be quantitatively inhibited by protein binding, showed a great...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 84bac2e0-92d4-5d1c-983e-9c5508bddcaf
DOI: 10.1007/s11426-011-4337-4

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Homogeneous label-free fluorescent assay of small molecule-protein interactions using protein binding-inhibited transcription nanomachine.DOI 10.1007/s11426-011-4337-4Read

A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applicationsSemantic proximity Read

Cell-free transcriptional regulation via nucleic-acid-based transcription factorsSemantic proximity Read

Solid-phase translation and RNA-protein fusion: a novel approach for folding quality control and direct immobilization of proteins using anchored mRNA.Semantic proximity Read

Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular moleculesSemantic proximity Read

Nucleic Acid-based Small Molecules as Targeted Transcription Therapeutics for ImmunoregulationSemantic proximity Read

Terminal protection of small molecule-linked DNA: A versatile biosensor platform for protein binding and gene typing assay.Semantic proximity Read

Fluorinated RNA origami enables serum-stable nanodevices for sensing and targetingSemantic proximity Read

Homogeneous time-resolved fluorescence quenching assay (TruPoint) for nucleic acid detection.Semantic proximity Read

A flow cytometry-based screen for synthetic riboswitches.Semantic proximity Read

A novel approach of cell-free peptide synthesis and SNPs detection using nano-circular single-stranded DNA.Semantic proximity Read

Combining SELEX screening and rational design to develop light-up fluorophore-RNA aptamer pairs for RNA tagging.Semantic proximity Read

A label-free signal amplification assay for DNA detection based on exonuclease III and nucleic acid dye SYBR Green I.Semantic proximity Read