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Article

Combining SELEX screening and rational design to develop light-up fluorophore-RNA aptamer pairs for RNA tagging.

ACS chemical biology - 19 Nov 2010

Lee Jungjoon, Lee Kyung Hyun, Jeon Jongho, Dragulescu-Andrasi Anca, Xiao Fei, Rao Jianghong

Abstract excerpt

We report here a new small molecule fluorogen and RNA aptamer pair for RNA labeling. The small-molecule fluorogen is designed on the basis of fluorescently quenched sulforhodamine dye. The SELEX (Systematic Evolution of Ligands by EXponential enrichment) procedure and fluorescence screening in E. coli have been applied to discover the aptamer that can specifically activate the fluorogen with micromolar binding...

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Identifiers

PMID: 20809562
DOI: 10.1021/cb1001894
PMCID: PMC3044212

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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