Article

Engineering circular guide RNA and CRISPR-Cas13d-encoding mRNA for the RNA editing of <i>Adar1</i> in triple-negative breast cancer immunotherapy

2025-07-22

Abstract excerpt

Clustered regularly interspaced short palindromic repeat Cas endonuclease (CRISPR-Cas) systems, such as RNA-editing CRISPR-Cas13d, are poised to advance the gene therapy of various diseases. However, their clinical development has been challenged by 1) the limited biostability of linear guide RNAs (lgRNAs) susceptible to degradation, 2) the immunogenicity of prokaryotic microorganism-derived Cas proteins in human...

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Literature Corpus work: 83d69440-d7b8-5c01-924b-dd418155f9ee
DOI: 10.1101/2025.07.22.666181

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