Article

Topical Eyedrop of 4-Hydroxytamoxifen Enables Cre-Mediated Gene Editing in Oligodendrocytes

2025-12-22

Abstract excerpt

Inducible CreERT-mediated gene editing is a powerful tool for targeted gene manipulation, but systemic tamoxifen (Tam) administration can cause off-target effects and toxicity. Here we demonstrate that topical administration of 4-hydroxytamoxifen (4-OHT) eye drops in Plp1-CreERT/tdTomatof/f mice effectively induces CreERT-mediated recombination specifically in oligodendrocytes of the optic nerve, optic chiasm, and...

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Literature Corpus work: 7cd38c03-8a0c-598a-baae-59af30a65298
DOI: 10.20944/preprints202512.1843.v1

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Topical Eyedrop of 4-Hydroxytamoxifen Enables Cre-Mediated Gene Editing in OligodendrocytesDOI 10.20944/preprints202512.1843.v1Read

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