Article

Construction of thyA deficient Lactococcus lactis using the Cre-loxP recombination system

2015-09-01

Abstract excerpt

Lactococcus lactis is widely used in the food industry as a food-grade non-pathogenic bacterium. In this study, we constructed the thymidylate synthase gene (thyA) deficient strain (NZ9000-3) derived from L. lactis NZ9000 using the Cre-loxP recombination system. Two loxP (lox66 and lox71) sites were integrated into the chromosome at target sites, and the thyA gene was replaced by a chloramphenicol resistance gene...

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Identifiers and source

Literature Corpus work: 7c24322b-c3b3-5815-8770-337b30801573
DOI: 10.1007/s13213-014-1005-x

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