Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis.

doi:10.1186/s12934-017-0770-1

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Article

Development and evaluation of an efficient heterologous gene knock-in reporter system in Lactococcus lactis.

Microbial cell factories - 18 Sept 2017

Lu Yifei, Yan Hongxiang, Deng Jiezhong, Huang Zhigang, Jin Xurui, Yu Yanlan, Hu Qiwen, Hu Fuquan, Wang Jing

Abstract excerpt

BACKGROUND: Lactococcus lactis is a food grade probiotics and widely used to express heterologous proteins. Generally, target genes are knocked into the L. lactis genome through double-crossover recombination to express heterologous proteins stably. However, creating marker-less heterologous genes knocked-in clones is laborious. In this study, an efficient heterologous gene knock-in reporter system was developed...

Topics

Chromosomes, Bacterial
Gene Knock-In Techniques
Genes, Reporter
Genetic Vectors
Lac Operon
Lactococcus lactis
Mutation
Phenotype
Probiotics
Temperature

Source

Open in PubMed Open full text

Identifiers

PMID: 28923077
DOI: 10.1186/s12934-017-0770-1
PMCID: PMC5604289

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