Article
Bacteriophage resistance of a deltathyA mutant of Lactococcus lactis blocked in DNA replication.
Applied and environmental microbiology - 1 Jun 2002
Pedersen Martin B, Jensen Peter R, Janzen Thomas, Nilsson Dan
Abstract excerpt
The thyA gene, which encodes thymidylate synthase (TS), of Lactococcus lactis CHCC373 was sequenced, including the upstream and downstream regions. We then deleted part of thyA by gene replacement. The resulting strain, MBP71 deltathyA, was devoid of TS activity, and in media without thymidine, such as milk, there was no detectable dTTP pool in the cells. Hence, DNA replication was abolished, and acidification by...
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