Article

Systematic conformation-to-phenotype mapping via limited deep-sequencing of proteins

2022-04-13

Abstract excerpt

<h4>Summary</h4> Non-native conformations drive protein misfolding diseases, complicate bioengineering efforts, and fuel molecular evolution. No current experimental technique is well-suited for elucidating them and their phenotypic effects. Especially intractable are the transient conformations populated by intrinsically disordered proteins. We describe an approach to systematically discover, stabilize, and puri...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: bdf3a332-495a-5bec-876f-f46013d52e11
DOI: 10.1101/2022.04.12.488104

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Systematic conformation-to-phenotype mapping via limited deep-sequencing of proteinsDOI 10.1101/2022.04.12.488104Read

Deep mutational scanning reveals a de novo disulfide bond and combinatorial mutations for engineering thermostable myoglobin.Semantic proximity Read

Deep Mutational Scanning Reveals a De Novo Disulfide Bond and Combinatorial Mutations for Engineering Thermostable MyoglobinSemantic proximity Read

Engineered pathways for correct disulfide bond oxidation.Semantic proximity Read

Large-scale discovery, analysis and design of protein energy landscapes.Semantic proximity Read

Decoding conformational heterogeneity across disordered proteomesSemantic proximity Read

Conformational ensembles of the human intrinsically disordered proteome: Bridging chain compaction with function and sequence conservationSemantic proximity Read

Use of site-directed cysteine and disulfide chemistry to probe protein structure and dynamics: applications to soluble and transmembrane receptors of bacterial chemotaxis.Semantic proximity Read

Dosa: A method to covalently barcode proteins for high throughput biochemistrySemantic proximity Read

Deep mutational scanning: a new style of protein science.Semantic proximity Read

Investigating the Molecular Mechanisms Behind Uncharacterized Cysteine Losses from Prediction of Their Oxidation State.Semantic proximity Read

A deep dive into degrader-induced protein-protein interfaces.Semantic proximity Read

Subdomain interactions foster the design of two protein pairs with ∼80% sequence identity but different folds.Semantic proximity Read