Article

Dosa: A method to covalently barcode proteins for high throughput biochemistry

2025-09-23

Abstract excerpt

Deep mutational scanning couples a protein’s activity to DNA sequencing for high throughput assessment of the effects of all single amino acid substitutions, but it largely uses indirect assays, like growth, as proxy for protein activity. Here, we covalently link variant proteins in vivo to an RNA barcode by fusing them to E. coli tRNA (m 5 U 54 ) methyltransferase TrmA (E358Q), which forms a covalent bond wit...

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Literature Corpus work: 7884e439-b57f-5c82-8706-4527de3e346a
DOI: 10.1101/2025.09.21.677650

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