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Article

The design and characterization of two proteins with 88% sequence identity but different structure and function.

Proceedings of the National Academy of Sciences of the United States of America - 17 Jul 2007

Alexander Patrick A, He Yanan, Chen Yihong, Orban John, Bryan Philip N

Abstract excerpt

To identify a simplified code for conformational switching, we have redesigned two natural proteins to have 88% sequence identity but different tertiary structures: a 3-alpha helix fold and an alpha/beta fold. We describe the design of these homologous heteromorphic proteins, their structural properties as determined by NMR, their conformational stabilities, and their affinities for their respective ligands: IgG...

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Identifiers

PMID: 17609385
DOI: 10.1073/pnas.0700922104
PMCID: PMC1906725

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