Article

The WPRE improves genetic engineering with site-specific nucleases

2017-04-12

Abstract excerpt

Inclusion of the woodchuck hepatitis virus post-transcriptional response element (WPRE) in the 3’ UTR of mRNA encoding zinc-finger or TALE nucleases results in up to a fifty-fold increase in nuclease expression and a several-fold increase in nuclease-modified chromosomes. Significantly, this increase is additive with the enhancement generated by transient hypothermic shock. The WPRE-mediated improvement is seen ac...

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Literature Corpus work: 770d5682-e43c-5a21-9ab3-892275d5772f
DOI: 10.1101/126904

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