Article

Short-Term and Highly Efficient Cas9 Expression System Using Adenovirus Vector and Site-Specific Recombinase Cre

2021-09-22

Abstract excerpt

Genome editing techniques such as CRISPR/Cas9 have both become common gene engineering technologies and have been applied to gene therapy. However, the problems of increasing the efficiency of genome editing and reducing off-target effects that induce double-stranded breaks at unexpected sites in the genome remain. In this study, we developed a novel Cas9 transduction system, Exci-Cas9, using an adenovirus vector...

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Literature Corpus work: 73af3512-19c8-5ae7-97e4-f48136753ae7
DOI: 10.21203/rs.3.rs-900410/v1

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