Article

A Chemoenzymatic Strategy for Site-Specific Glyco-Tagging of Native Proteins for the Development of Biologicals

2024-08-13

Abstract excerpt

<h4>ABSTRACT</h4> Glycosylation is an attractive approach to enhance biological properties of pharmaceutical proteins, however, precise installation of glycans for structure-function studies remains challenging. Here, we describe a chemoenzymatic methodology for glyco-tagging of proteins by peptidoligase catalyzed modification of the N -terminus of a protein with a synthetic glycopeptide ester having an N -acet...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 6e5cc224-abc1-59b9-89ee-1b7490c65000
DOI: 10.1101/2024.08.13.607754

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

A Chemoenzymatic Strategy for Site-Specific Glyco-Tagging of Native Proteins for the Development of BiologicalsDOI 10.1101/2024.08.13.607754Read

Review of Glycosylation Engineering of Biopharmaceuticals: Methods and Protocols : A book edited by Alain BeckSemantic proximity Read

Glycosyltransferases as versatile tools to study the biology of glycansSemantic proximity Read

Multi-Tag: A modular platform of bioorthogonal probes for multi-modal (glyco)protein analysisSemantic proximity Read

Glycosylation Shapes the Efficacy and Safety of Diverse Protein, Gene and Cell TherapiesSemantic proximity Read

Glycosylation shapes the efficacy and safety of diverse protein, gene and cell therapiesSemantic proximity Read

Efficient Preparation and Bioactivity Evaluation of Glycan-Defined Glycoproteins.Semantic proximity Read

Metabolic precision labeling enables selective probing of O-linked <i>N</i> -acetylgalactosamine glycosylationSemantic proximity Read

Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research.Semantic proximity Read

A library of structurally homogeneous human <i>N</i> -glycans synthesized from microbial oligosaccharide precursorsSemantic proximity Read

Direct identification of nonreducing GlcNAc residues on N-glycans of glycoproteins using a novel chemoenzymatic method.Semantic proximity Read

Efficient transfer of sialo-oligosaccharide onto proteins by combined use of a glycosynthase-like mutant of Mucor hiemalis endoglycosidase and synthetic sialo-complex-type sugar oxazoline.Semantic proximity Read

Precision Glycoform Engineering: Combining plant and <i>in vitro</i> systems for tailored biopharmaceutical productionSemantic proximity Read