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Article

Generating and characterizing a comprehensive panel of CHO cells glycosylation mutants for advancing glycobiology and biotechnology research.

Scientific reports - 4 Oct 2024

Haryadi Ryan, Chan Kah Fai, Lin Pao Chun, Tan Yun Lei, Wan Corrine, Shahreel Wahyu, Tay Shi Jie, Nguyen-Khuong Terry, Walsh Ian, Song Zhiwei

Abstract excerpt

This report describes the development and characterization of a comprehensive collection of CHO cell glycosylation mutants with significant potential for advancing glycobiology and biotechnology. EPO-Fc and trastuzumab, two model molecules, were produced using these mutants to assess the effects of mutated glycogenes, and LC-MS/MS analysis was employed to quantitatively analyse their N-glycans. EPO-Fc exhibited...

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Identifiers

PMID: 39367021
DOI: 10.1038/s41598-024-73722-z
PMCID: PMC11452509

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