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Article

Direct identification of nonreducing GlcNAc residues on N-glycans of glycoproteins using a novel chemoenzymatic method.

Bioconjugate chemistry - 1 Jan 2000

Boeggeman Elizabeth, Ramakrishnan Boopathy, Kilgore Charlton, Khidekel Nelly, Hsieh-Wilson Linda C, Simpson John T, Qasba Pradman K

Abstract excerpt

The mutant beta1,4-galactosyltransferase (beta4Gal-T1), beta4Gal-T1-Y289L, in contrast to wild-type beta4Gal-T1, can transfer GalNAc from the sugar donor UDP-GalNAc to the acceptor, GlcNAc, with efficiency as good as that of galactose from UDP-Gal. Furthermore, the mutant can also transfer a modified sugar, C2 keto galactose, from its UDP derivative to O-GlcNAc modification on proteins that provided a functional...

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Identifiers

PMID: 17370997
DOI: 10.1021/bc060341n
PMCID: PMC3534963

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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