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Why Standard LP-Based Flux Balance Analysis Cannot Detect Synergy for Essential Gene Pairs in ESKAPE Pathogens: A Systematic Evaluation and Proof-of-Concept Feature-Based ML Alternative

2026-04-20

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<title>Abstract</title> <p>Background. Flux balance analysis (FBA) is widely used to simulate gene essentiality in genome-scale metabolic models (GEMs), and several studies have proposed using FBA-based combination simulations to predict antibiotic synergy. However, whether standard LP-based FBA can detect synergistic interactions between essential gene pairs -- as opposed to merely confirming individual gene ess...

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Literature Corpus work: 6dbcf88e-bb67-5be6-a03b-05ab578a8bba
DOI: 10.21203/rs.3.rs-9398278/v1

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