Article
Generation of chromosomal deletions in dicotyledonous plants employing a user-friendly genome editing toolkit.
The Plant journal : for cell and molecular biology - 1 Jan 2017
Ordon Jana, Gantner Johannes, Kemna Jan, Schwalgun Lennart, Reschke Maik, Streubel Jana, Boch Jens, Stuttmann Johannes
Abstract excerpt
Genome editing facilitated by Cas9-based RNA-guided nucleases (RGNs) is becoming an increasingly important and popular technique for reverse genetics in both model and non-model species. So far, RGNs were mainly applied for the induction of point mutations, and one major challenge consists in the detection of genome-edited individuals from a mutagenized population. Also, point mutations are not appropriate for...
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