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Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention.

Plant molecular biology - 1 Mar 2018

Zhang Qiang, Xing Hui-Li, Wang Zhi-Ping, Zhang Hai-Yan, Yang Fang, Wang Xue-Chen, Chen Qi-Jun

Abstract excerpt

KEY MESSAGE: We present novel observations of high-specificity SpCas9 variants, sgRNA expression strategies based on mutant sgRNA scaffold and tRNA processing system, and CRISPR/Cas9-mediated T-DNA integrations. Specificity of CRISPR/Cas9 tools has been a major concern along with the reports of their successful applications. We report unexpected observations of high frequency off-target mutagenesis induced by...

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Identifiers

PMID: 29476306
DOI: 10.1007/s11103-018-0709-x
PMCID: PMC5978904

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