Article

S2′ cleavage site plays a decisive role in expansion of Vero cell tropism by infectious bronchitis virus HV80, with Q855H promoting cell-to-cell fusion

2023-04-19

Abstract excerpt

<h4>ABSTRACT</h4> Infectious bronchitis virus (IBV) has restricted cell tropism. Apart from the Beaudette strain, other IBVs cannot infect mammalian cell lines. The limited cell tropism of other IBVs has hindered the development of IBV vaccines and research on mechanisms of IBV infection. In a previous study, a new Vero-cell-adapted strain HV80 was obtained via serial chicken embryo and cell passaging of strain H...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 694a465c-c73d-5a17-98d1-5fe56387cb12
DOI: 10.1101/2023.04.19.537537

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

S2′ cleavage site plays a decisive role in expansion of Vero cell tropism by infectious bronchitis virus HV80, with Q855H promoting cell-to-cell fusionDOI 10.1101/2023.04.19.537537Read

Two mutations on S2 subunit were critical for Vero cell tropism expansion of infectious bronchitis virus HV80.Semantic proximity Read

Accumulation of mutations in nsp4, E, and the S2 subunit underlies mammalian cell tropism expansion and virulence attenuation of avian coronavirus.Semantic proximity Read

Disruption of the S1/S2 multibasic cleavage site attenuates infectious bronchitis virus, while S2' partially restores viral virulence and expands tissue tropism.Semantic proximity Read

The V577S mutation in the S2 subunit of QX-type IBV attenuates viral adaptation to chicken oviduct epithelial cells and pathogenicity in laying hens.Semantic proximity Read

The N545S and K717N substitution at the N-glycosylation sites of the S2 subunit of avian infectious bronchitis virus can significantly enhance viral pathogenicitySemantic proximity Read

Acquisition of Cell–Cell Fusion Activity by Amino Acid Substitutions in Spike Protein Determines the Infectivity of a Coronavirus in Cultured CellsSemantic proximity Read

Respiratory syncytial virus entry mechanism in host cells: A general overviewSemantic proximity Read

Adaptive truncation of the S gene in IBV during chicken embryo passaging plays a crucial role in its attenuation.Semantic proximity Read

Characterization of the complete genome, antigenicity, pathogenicity, tissue tropism, and shedding of a recombinant avian infectious bronchitis virus with a ck/CH/LJL/140901-like backbone and an S2 fragment from a 4/91-like virus.Semantic proximity Read

Rapid reconstruction of infectious bronchitis virus expressing fluorescent protein from its nsp2 gene based on transformation-associated recombination platformSemantic proximity Read

Two mutations in the HR2 region of Newcastle disease virus fusion protein with a cleavage motif "RRQRRL" are critical for fusogenic activity.Semantic proximity Read

The S2 Subunit of QX-type Infectious Bronchitis Coronavirus Spike Protein Is an Essential Determinant of Neurotropism.Semantic proximity Read