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Multiplexed CRISPR gene editing in primary human islet cells with Cas9 ribonucleoprotein

7 Dec 2023

Abstract excerpt

Successful genome editing in primary human islets could reveal features of the genetic regulatory landscape underlying β cell function and diabetes risk. Here, we describe a CRISPR-based strategy to interrogate functions of predicted regulatory DNA elements using electroporation of a complex of Cas9 ribonucleoprotein (Cas9 RNP) and guide RNAs into primary human islet cells. We successfully targeted coding regions...

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PMID: 38205242
DOI: 10.1016/j.isci.2023.108693

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