Article

Quantitative comparison of single-cell RNA sequencing versus single-molecule RNA imaging for quantifying transcriptional noise

2024-08-10

Abstract excerpt

<h4>SUMMARY</h4> Stochastic fluctuations (noise) in transcription generate substantial cell-to-cell variability. However, how best to quantify genome-wide noise, remains unclear. Here we utilize a small-molecule perturbation (IdU) to amplify noise and assess noise quantification from numerous scRNA-seq algorithms on human and mouse datasets, and then compare to noise quantification from single-molecule RNA FISH (...

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Literature Corpus work: 66a8994c-adfa-5c3c-a8f0-d79f1ac45f8f
DOI: 10.1101/2024.08.09.607289

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