Article

Identification of high confidence human poly(A) RNA isoform scaffolds using nanopore sequencing

2020-11-20

Abstract excerpt

<h4>ABSTRACT</h4> Nanopore sequencing devices read individual RNA strands directly. This facilitates identification of exon linkages and nucleotide modifications; however, using conventional methods the 5′ and 3′ ends of poly(A) RNA cannot be identified unambiguously. This is due in part to the architecture of the nanopore/enzyme-motor complex, and in part to RNA degradation in vivo and in vitro that can obscur...

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Literature Corpus work: 30e3b818-1247-5e19-afd3-07d02edcb054
DOI: 10.1101/2020.11.18.389049

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