Article

Nanopore native RNA sequencing of a human poly(A) transcriptome: RNA extraction, cDNA conversion and direct RNA and cDNA library preparation for Oxford Nanopore

2019-08-13

Abstract excerpt

<title>Abstract</title> <p>High throughput cDNA sequencing technologies have dramatically advanced our understanding of transcriptome complexity and regulation. However, these methods lose information contained in biological RNA because the copied reads are often short and because modifications are not carried forward in cDNA. We address these limitations using a native poly(A) RNA sequencing strategy developed b...

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Literature Corpus work: 56789357-dd3b-5ee3-b9c3-8e1e57d6f343
DOI: 10.21203/rs.2.12872/v1

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