Article

JUNO-Coated Beads as a Functional Assay to Capture and Characterize Fertilization-Competent Human Sperm

2025-10-01

Abstract excerpt

<h4>Study question</h4> Can human fertilization-competent spermatozoa be captured through their ability to bind the oocyte receptor JUNO? <h4>Summary answer</h4> JUNO-coated beads, which mimic the oocyte geometry, selectively bound acrosome-reacted spermatozoa with intact DNA, revealing that vitrification preserves functional sperm binding while slow cryopreservation increases non-specific interactions. <h4>What...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 64c757d3-e6e0-5494-9034-bdb9b2813db6
DOI: 10.1101/2025.09.30.679507

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

JUNO-Coated Beads as a Functional Assay to Capture and Characterize Fertilization-Competent Human SpermDOI 10.1101/2025.09.30.679507Read

Human Sperm-Induced Cell-Cell Fusion Requiring JUNO (hSPICER): A paradigm shift to test sperm fertilizing potentialSemantic proximity Read

An integrated AI-microfluidic platform reveals the broad persistence and developmental potential of rare sperm in non-obstructive azoospermiaSemantic proximity Read

Genetic Diversity in the IZUMO1-JUNO Protein-Receptor Pair Involved in Human ReproductionSemantic proximity Read

Molecular architecture of the human sperm IZUMO1 and egg JUNO fertilization complexSemantic proximity Read

Improving sperm selection strategies for assisted reproduction through closing the knowledge gap in sperm maturation mechanicsSemantic proximity Read

JUNO, the receptor of sperm IZUMO1, is expressed by the human oocyte and is essential for human fertilisationSemantic proximity Read

Identifying Pre-operative Factors for Successful Micro-dissection Testicular Sperm Extraction in Non-Obstructive AzoospermiaSemantic proximity Read

Structure of IZUMO1-JUNO reveals sperm-oocyte recognition during mammalian fertilization.Semantic proximity Read

A conserved fertilization complex bridges sperm and egg in vertebratesSemantic proximity Read

MAIA, Fc receptor–like 3, supersedes JUNO as IZUMO1 receptor during human fertilizationSemantic proximity Read

Sperm-Egg Fusion: A Molecular Enigma of Mammalian ReproductionSemantic proximity Read

Evolution of the WHO “Semen” processing manual from the first (1980) to the sixth edition (2021)Semantic proximity Read