Article

High-throughput competitive binding assay for targeting RNA with small molecules: discovery of new PreQ <sub>1</sub> riboswitch ligands

2024-05-22

Abstract excerpt

In the evolving landscape of RNA targeting, there is an indisputable need for new screening methodologies to find small molecules targeting relevant tertiary RNA structures, like viral pseudoknots or bacterial riboswitches. Here, we developed a competitive binding high-throughput screening assay to identify ligands for the bacterial PreQ 1 -I riboswitch. In this assay, ligands compete with a rationally designed q...

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Literature Corpus work: 633b1bf2-b9ad-5dd1-b567-678a96400ddd
DOI: 10.1101/2024.05.22.595132

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High-throughput competitive binding assay for targeting RNA with small molecules: discovery of new PreQ <sub>1</sub> riboswitch ligandsDOI 10.1101/2024.05.22.595132Read

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