Article

Tissue-specific tagging of endogenous loci in <i>Drosophila melanogaster</i>

2015-08-28

Abstract excerpt

Fluorescent protein tags have revolutionized cell and developmental biology, and in combination with binary expression systems they enable diverse tissue-specific studies of protein function. However these binary expression systems often do not recapitulate endogenous protein expression levels, localization, binding partners, and developmental windows of gene expression. To address these limitations, we have devel...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 606d5fb4-4194-5ea1-a3b6-1226e06e35b7
DOI: 10.1101/025635

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Tissue-specific tagging of endogenous loci in <i>Drosophila melanogaster</i>DOI 10.1101/025635Read

Expanded FLP toolbox for spatiotemporal protein degradation and transcriptomic profiling in <i>C. elegans</i>Semantic proximity Read

Functional tagging of endogenous proteins and rapid selection of cell pools (Rapid generation of endogenously tagged <i>piwi</i> in ovarian somatic sheath cells.)Semantic proximity Read

Characterization of Endogenous and Epitope-Tagged Ubiquitin Expression and Ubiquitination Patterns at <i>Drosophila</i> Neuromuscular JunctionsSemantic proximity Read

Tissue-specific downregulation of <i>EDTP</i> suppresses polyglutamine protein aggregates and extends lifespan in <i>Drosophila</i>Semantic proximity Read

Harnessing fusion of genome-edited human stem cells to rapidly screen for novel protein functions <i>in vivo</i>Semantic proximity Read

Sleeping ORANGE: A Transposase-Enhanced CRISPR Approach to Boost Endogenous Protein Tagging EfficiencySemantic proximity Read

Quantitative proteomic analysis of Parkin substrates in Drosophila neurons.Semantic proximity Read

Endoplasmic Reticulum Proteins Impact Penetrance in a <i>Pink1</i>-Mutant <i>Drosophila</i> ModelSemantic proximity Read

Cell type-specific biotin labeling <i>in vivo</i> resolves regional neuronal proteomic differences in mouse brainSemantic proximity Read

An <i>in vivo</i> platform to identify pathogenic lociSemantic proximity Read

Cre-ating somatic cell genetic mosaics in the mouse.Semantic proximity Read

EyaHOST, a modular genetic system for investigation of intercellular and tumor-host interactions <i>in Drosophila melanogaster</i>Semantic proximity Read